ZORBAX Reversed-Phase Columns | Agilent (2024)

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ZORBAX Reversed-Phase Columns | Agilent (1)

The ZORBAX column family is one of the most popular high-performance liquid chromatography (HPLC) column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution. From Eclipse Plus, Eclipse XDB, StableBond, Bonus-RP and more, the ZORBAX column portfolio offers a wide selection of stationary phases allowing you to fine-tune your selectivity to match your application. Improve resolution by utilizing sub-2 µm particles to maximize system efficiency and confidently scale-up to preparative HPLC and/or easy HPLC method transfer.

  • Reversed-Phase LC Columns | Liquid Chromatography

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ZORBAX Reversed-Phase Columns | Agilent (2)

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Product Details

Features

  • Traditional, fully porous particles available in 1.8, 3.5, and 5 µm sizes. With selected products also available in 7 μm particles for preparative HPLC (see Prep LC columns section)
  • ZORBAX Eclipse Plus columns are the recommended starting point for method development at mid-pH and benefit from the ultimate performance from fully porous silica-based columns
  • ZORBAX StableBond columns offer the highest stability for low pH applications down to pH 1 while ZORBAX SB-Aq deliver robust, reproducible separations with highly aqueous mobile phases, including 100% water
  • ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns are eXtra Densely Bonded (XDB) and double endcapped through a proprietary process to cover as many active silanols as possible. Eclipse XDB columns are available in 1.8, 3.5, 5 and 7 μm particle sizes for high speed, high resolution, analytical and prep scale separations
  • ZORBAX Extend-C18 columns offer high efficiency and selectivity advantages for basic compounds in for high pH applications up to pH 11.5
  • ZORBAX Bonus RP columns offer unique selectivity due to an embedded amide linkage in the C14-alkyl chain making them ideal for 100% aqueous mobile phases and for compounds that are not retained on standard reverse phase columns
  • ZORBAX Rx columns are made from highly pure (>99.995 %) porous silica with a higher carbon load (12%)
  • ZORBAX Eclipse PAH columns deliver high resolution separation of the 16 polycyclic aromatic hydrocarbons (PAHs) in EPA 610 and have been specifically tested with PAHs for maximum reproducibility
  • ZORBAX Rapid Resolution High Definition (RRHD) columns are packed with 1.8 μm particles and stable up to 1200 bar for fast, high-resolution separations of your most complex samples
  • ZORBAX Rapid Resolution High Throughput (RRHT) columns use totally porous 1.8 μm particles to provide maximum resolution in ultra-fast and high-resolution analyses with a pressure limit of 600 bar
  • Agilent UHPLC Guards extend the lifetime of analytical columns - without diminishing performance - and use easy-to-install hardware that fits directly on the end of the column

Tools

A series of tools to help you navigate to the best HPLC column choice for your application.

    Captiva Syringe Filter Selector Tool

    The Captiva Syringe Filter selector tool helps you find the best syringe filter to suit your sample prep needs.

      Solid Phase Extraction (SPE) Selector Tool

      The SPE Selector Tool helps find the best SPE based on your application, SPE format, sample volume and bed mass.

        Essential Supplies Guide for InfinityLab LC Series Instruments

        Discover the right supplies for your instrument to achieve peak performance and optimize your LC workflow.

          How It Works

          Applications

          Videos

          Literature

          Key Literature

          A Proven and Reliable Portfolio of Totally Porous HPLC Columns

          The Agilent ZORBAX family offers all advantages of totally porous particle columns such as increased retention, loadability and resistance to sample solvents.

          • Posters
          • English
          • 28 Jul 2020
          • 2.76 MB
          • PDF

          A Comprehensive Workflow for Analysis of PFAS in Wastewater per EPA Method 1633

          This application note demonstrates a comprehensive workflow, including sample preparation, consumables, data acquisition/analysis, and reporting for PFAS listed in EPA Method 1633 Draft 4 and in E.U. and U.K. regulations.

          • Application Notes
          • English
          • 16 Nov 2023
          • 644.50 KB
          • PDF

          Analysis of Per- and Polyfluoroalkyl Substances (PFAS) in Solid Samples

          In this application note, the Agilent Bond Elut PFAS WAX 150 mg, 6 mL cartridge and Carbon S was used for the extraction of PFAS from soil by following the EPA draft method 1633 protocol.

          • Application Notes
          • English
          • 23 Jan 2023
          • 416.77 KB
          • PDF
          Application Notes

          Related Products

          News

          Acquires Polymer Standards Service, Broadening Offerings for GPC/SEC Polymer Analysis

          Agilent acquires Polymer Standards Service (PSS), broadening offerings for gel permeation chromatography (GPC) and size exclusion chromatography (SEC) polymer analysis. The acquisition expands Agilent’s portfolio and offerings for the analysis of natural and synthetic polymers.

          • 02 Aug 2022

          PFAS Analysis in Milk, Infant Formula, and Related Ingredients Using HPLC

          Per- and polyfluoroalkyl substances (PFAS) are a large group of anthropogenic chemicals that have been applied in industrial, commercial, and domestic products since the 1950s. Because of their toxicity, persistence, and bioaccumulation potential, PFAS have become global environmental pollutants. Besides the environment, the food chain represents another source of exposure, and the risk related to the presence of PFAS in foods has become of increased interest. Whole milk, infant formula, and ingredients used in infant formula production represent important foodstuffs that require sensitive methods with reporting limits at low parts per billion levels or lower for multiple PFAS. This article summarizes optimization experiments and validation of a complete workflow, including sample preparation and a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for determination of sixteen priority PFAS analytes listed by the U.S. Food and Drug Administration (FDA).

          • 07 Jan 2022

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          ZORBAX Reversed-Phase Columns | Agilent (2024)
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